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To make excitatory neurons or astrocytes sensitive to blue light, 1 h after plating (DIV 0 and DIV 2 for neurons and astrocytes, respectively) Ngn2 -hiPSCs or astrocytes were transduced with an adeno-associated virus (AAV) serotype 2 encoding Channelrhodopsin-2 (ChR2) (AAV2-hSyn-hChR2(H134R)-mCherry, UNC Vector Core) 74 and AAV serotype 2 encoding ChR2 (AAV-GFAP-hChR2(H134R)-mCherry, UNC Vector Core) 44,75 , respectively
A plain B12 (cyanocobalamin or methylcobalamin) shot supplies vitamin B12 to support normal energy metabolism
A dual AAV system enables the Cas9-mediated correction of a metabolic liver disease in newborn mice
Pinpointing Absorption Issues: Testing helps us determine if your body is failing to absorb B12 from food due to gut issues, age, or autoimmune conditions like pernicious anemia
2b) or when richness and evenness were combined in the ShannonWiener Diversity Index H (Fig
