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above approximately pH 7.4 the Cu(II) chelate begins to release copper and the solution darkens
Each vial contains sterile water with 0.9% benzyl alcohol to help inhibit microbial growth during repeated laboratory handling under appropriate sterile conditions
According to the NHS, untreated deficiency can affect the nervous system and blood health
The vials were placed in a Luminometer 1251 (LKB Wallac, Turku, Finland) and allowed to equilibrate in the dark for 5 minutes at 37C, with intermittent shaking, and then the background light output was recorded in millivolts
The way B12 deficiency is treated varies from country to country
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