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Some 10 pl of DNA mixture containing Rosa26 -CTV-GK plasmid (20 ng/L final concentration), SpCas9 mRNA (120 ng/L final concentration), and Rosa26 sgRNA (100 ng/L), was microinjected into each of the zygotes and were cultured in KSOM medium at 37 C in a humidified incubator containing 5% CO 2 for 16 h

[1] [2] Each pen should be disposed of properly after a single use, even if medication remains visible in the cartridge

L-carnitine is a substance that supports the conversion of fatty acids into energy

Pancreas 35, e10e17

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