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The oxidized nucleosides are detected in the urine and their content has been related to the total oxidative stress of an organism
Swirl gently until clear, and do not shake the copper-tinted solution
PIT SWEEPER PACKAGE (OPTIONAL) A PVC pit sweeper is installed in the pit to keep particles suspended and swept toward pump suction for removal
Keep simple logs of injection timing, sites, doses, and any effects noticed
siRNA silencing MODE-K IECs were seeded on 6-well plates at ~70% confluence for siRNA silencing with either Gpx4 siRNA ( siGpx4 , Ambion, s122098), Alox12 ( siAlox12 , Ambion, s62261), Alox15 ( siAlox15 , Ambion, s62271) or scrambled control siRNA (siCtrl, Ambion 4390843) and RNAiMAX (Thermo Fisher Scientific, 13778100) transfection over 48 h unless otherwise indicated, according to the recommended protocol
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