For the immunohistochemical technique, 3 m sections were done of each selected paraffin block, using the Envision System (Dako, USA), following standardized protocol with adequate controls and using the following antibodies: S100 protein, HMB45, Neuron specific enolase (NSE), Cytokeratin AE1-AE3 (CKAE1-AE3), (GFAP), chromogranin, synaptophysin and p53
Even if we get to the point that medications alone can help people with obesity lose weight while preserving muscle, being in the normal weight range with a good muscle-to-fat ratio doesnt guarantee good health as much as we might wish it werent true, a better diet and more exercise might still be necessary
10.1159/000183292 Nephron
doi: 10.1200/JCO.2016.67.4044 205 YuHRohanT
Supplementary Information Additional file 1: Figure S1 (download DOCX