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Next, they were washed in PTwH (100 PBS 10, 2 ml Tween 20, 1 ml of 10 mg ml 1 heparin stock solution) for 1 10 min, 1 20 min, 1 30 min, 1 1 h, 1 2 h and 1 2 days
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RIPK1 undergoes phosphorylation instead of ubiquitination, triggering the activation of RIPK3, which subsequently phosphorylates the mixed lineage kinase domain-like pseudokinase (MLKL)
Sexton FM, Maize JC
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