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Then, all animals were euthanized by bleeding after transection of the abdominal vena cava under anesthesia and the liver, heart, soleus muscle, gastrocnemius muscle, tibialis anterior muscle, extensor digitorum longus muscle, epididymal white adipose tissue, and brown adipose tissue were harvested and immersed in RNAlater solution (Qiagen, Venlo, Netherlands) for qPCR analysis

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The thin outside, fat inside (TOFI) phenomenon affects a significant portion of the population, particularly as people age

This hypothetical mechanism distinguishes Ipamorelin from other ghrelin mimetics

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