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Immunofluorescence tissues were mounted with Immuno-Mount (Thermo Fisher, 9990402) and coverslipped for imaging
This assay quantified social competence and cognitive flexibility by measuring exploration time toward unfamiliar conspecifics (Stranger 1/2) versus empty cages/objects
coli BL21(DE3) cys cells cotransformed with plasmids pCGPx3M and pMazF (TaKaRa) were grown at 37C in 400 mL of MM medium (modified M9 minimal medium) supplemented with ampicillin (100 g/mL), kanamycin (25 g/mL), chloramphenicol (25 g/mL) and Cys (50 g/mL) on a shaker
Vitamin C mechanisms involve serving as enzymatic cofactor for collagen hydroxylation and providing direct antioxidant protection, creating effects requiring ongoing application for maintaining antioxidant benefits but potentially supporting durable collagen improvements if used sufficiently long
Significant findings and lessons were learned from this pilot phase
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