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Screening was performed with a TGR assay using DTNB, including a 15 min preincubation of recombinant enzyme, inhibitor, and NADPH before addition of substrate (Table 3)
AOD-9604 Assay Design Considerations When incorporating AOD-9604 into a new lipolysis assay, researchers commonly include vehicle controls, a well-characterized positive control such as isoproterenol, and a dose range spanning two to three orders of magnitude
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