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2.8 Immunoblot analysis Total protein was isolated from PIG1 and PIG3V cells disrupted in RIPA lysis buffer (Beyotime, China) with protease and phosphatase inhibitor cocktails, and quantified via BCA assay (Beyotime, China)

I kept it in the refrigerator and used it for half a day and it certainly brightened the skin tone and the lightness softened a lot.The unique aroma and stickiness is a shame but the whitening effect is great enough to use

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