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Refrigeration is the correct storage method
After 1216 days post seeding, membrane inserts containing confluent monolayer of Caco-2 cells were transferred into Ussing chambers 47,48
The goal here is no longer rapid replenishment but steady upkeep
You cant just put the sealed container in your household trash
Cell-mediated immunity primarily needs protein antigen degradation in the endocytic vesicles of antigen presenting cells (macrophages, dendritic cells), to be able to present smaller peptides on the cell surface through major histocompatibility complex antigens to activate antigen-specific T cell proliferation
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