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Sci Rep 14 , 7487 (2024)

Complementary DNA (cDNA) was made by reverse transcription of RNA (2 g) using Moloney murine leukemia virus reverse transcriptase (Promega, Madison, MI, USA) and labeled with Biotin-16-2-deoxy-uridine-5-triphosphate (Roche Diagnostics, Indianapolis, IN, USA)

Neurological symptoms range from impaired cognitive function, depression, seizures, and premature aging of neurons, to paresthesias, painful dysesthesias, and thermal sensations (6, 11, 16, 58)

Sample sizes per experimental group were chosen based on previously reported similar studies [4, 5]

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