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We initiated an evolution campaign with seven PANCE passages on GD_M, followed by 12 passages of high-stringency dual selection penalizing proteases that cleave VAMP1, and concluded with 16 passages on a circuit using a weaker promoter in front of the GD_M substrate to increase positive selection stringency (Extended Data Fig
Mercury exposure and health effects: what do we really know
12 In addition, NACA prevented acetaminophen-induced cataractogenesis in rats
doi:10.1055/s-0040-1718965
The honest answer is: we do not know
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