In OGD/R-treated PC12 cells, CAL (60 M for 24 h) enhanced cell survival, reduced apoptosis (as shown by Annexin V-PI staining), and decreased lipid ROS, iron accumulation, and oxidative stress markers, ultimately improving cell viability (Liu H
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In cell culture studies, concentrations of 10 to 1,000 nanomolar (nM) are used, with the physiological range at approximately 200 nM
One must consult with a dermatologist before taking the injection
After freezing, samples were homogenized using Retsch MM301 ball mills (Retsch Gmbh, Germany) at 30 Hz for 10 min and then kept at 80 C overnight