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Relative expression levels were determined by real-time PCR on a Bio-Rad CFX Connect or Real-Time System (CFX96 Real-Time System) using SYBR Green or the PowerUp SYBR Green kit (Thermo Fisher Scientific), and the primer sequences are listed in Supplemental Methods
Following the exclusion of outliers from the data set in sensitivity analyses, the observed changes in weight, body mass index (BMI) and waist circumference remained statistically significant
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This is a meaningful data set, and digging into the distribution tells a more nuanced story than the headline number
It most commonly affects people older than 60 and with a history of arterial disease
