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Too acidic and the copper dissociates
This timeline matches B12 pharmacokinetics: intramuscular methylcobalamin reaches peak serum concentration at 8-12 hours, but tissue saturation (particularly in the liver and nervous system) occurs over 48-72 hours as the vitamin is transported into cells and converted to active coenzyme forms (Andrs et al., 2018)
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Inject bacteriostatic water slowly along the inner wall of the vial, swirl gently, and refrigerate the solution at 28 C
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