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By pre-treating rats with the non-specific cholinergic receptor antagonist atropine as well as the specific M3 cholinergic receptor antagonist 4-DAMP and performing vagotomy, the researchers found that atropine and 4-DAMP significantly reduced metformin-induced GLP-1 secretion, whereas vagotomy had no effect on metformin-induced GLP-1 secretion

Polat BE, Hart D, Langer R, Blankschtein D

The injections can be self-performed or performed by a medical professional

Moreover, correlation analysis was performed between identified differential ACs and serum ALT (Table 1), demonstrating that increased ACs are significantly and positively correlated to the severity of abnormal liver function ( p 0.05, Figure 7 A), which may explain the absence of changes in SCACs and MCACs in the ALF group compared with the NLF group

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