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Antigen retrieval was performed by high-pressure heating of slides in sodium citrate buffer (pH 6.0) or 1 mM EDTA buffer for 5 minutes
Cysteine is often a limiting factor to GSH production, therefore, supplementing with L-cysteine can be used to improve GSH status in the body and has also been associated with lower levels of inflammation and insulin resistance
Moreover, in studies it appears to support angiogenesis and tendon cell models
Although current evidence is limited to osteosarcoma models, the observed effectiveness of 17-AAG in inhibiting stress-related survival signaling provides a rationale for exploring its potential as a senolytic candidate for targeting SnCs in companion dogs
