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In brief, an aliquot (200 mg) of each sample was suspended, while frozen, in a solution containing 500 l of freshly prepared extraction buffer (200 mM Tris, 200 mM NaCl, 20 mM EDTA, pH of 8.0), 200 l of 20% SDS and 500 l of a mixture of phenol:chloroform:isoamyl alcohol (25:24:1) in 2 ml VK05 Precellys tubes containing 0.5 mm glass beads (Bertin Technologies)

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The Prohibited List , Section S2.2.4 Growth Hormone Releasing Factors

It is a synthetic fragment derived from a protein found in human gastric juice, where researchers originally studied it for its protective effects on the gastrointestinal lining

Nassar ZD, Mah CY, Dehairs J, Burvenich IJ, Irani S, Centenera MM, et al