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Using sterile water means every needle puncture after the first one increases your contamination risk with zero bacterial defense in the solution
[12] For most people with dietary insufficiency, oral supplementation is effective, as even in the absence of intrinsic factor, approximately 1% of B12 can be absorbed through passive diffusion
Following this, they were washed three times with PBST (1X PBS and 0.05% Tween-20), then incubated with mouse IgG FITC conjugated secondary antibody (Invitrogen)
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