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The SOD activity of canola root showed a similar trend to that observed in canola shoot (Fig
Brechbuhl HM, Gould N, Kachadourian R, Riekhof WR, Voelkerand DR, Day BJ
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Cell pellets were resupended in ice-cold buffer 1 containing 20 mM imidazole, 50 mM Na x H y PO 4 , 300 mM NaCl, pH 8.0 at 4 C and stirred on ice with DNase I and 10 mg/mL lysozyme for 1 h before sonication
These alterations greatly decrease its ability to bind to IGF-binding proteins, and it is this property that allows LR3 to stay active in the bloodstream longer than native IGF-1
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