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cDNA was prepared from total RNA and used for gene expression by real-time quantitative RTPCR (qPCR) as described [53] using TaqMan Gene Expression Assays (ThermoFischer Scientific) specific for human GCLC (Hs00155249_m1), OCT4 (Hs00999632_g1), and ACTB (Hs01060665_g1)

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doi: 10.1038/nrendo.2015.129 27 FeldmanELNaveKAJensenTSBennettDLH

Specific Dosage Guidelines Severe Deficiency : For patients with severe vitamin B12 deficiency, an initial dose of 1 mg (1000 g) of intramuscular hydroxocobalamin is commonly administered

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