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Semaglutide is a glucagon-like peptide-1 (GLP-1) receptor agonist approved for type 2 diabetes management, chronic weight management, and in the case of Wegovy, cardiovascular risk reduction in certain patients

Pellets were then resuspended in 50 l of density buffer reaction and combined with 1 g of library plasmid

Cell lysates were centrifuged at 12,000 g for 10 minutes, and supernatants added to 40 L anti-FLAG M2 beads (Sigma Aldrich) or anti-HA Magnetic beads (Thermo Fisher Scientific) and incubated at 4C overnight

Even on a deficit

Cellular uptake studies revealed a 5.3-fold increase in drug internalization in Caco-2 cells when encapsulated in nanoparticles