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For gel electrophoresis, the immunoprecipitates were resuspended in reducing loading buffer (Novex, Life Technologies, Carlsbad, CA, USA) with TCEP (Pierce, Thermo Scientific, IL, USA) and the proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes (Life Technologies)
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Weight loss during this phase is modest, typically 1 to 3% of body weight over four weeks
12% of Britons and 6% of Americans noticed more regular menstrual cycles, suggesting a potential regional difference in how GLP-1s impact hormones
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