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One of the central regulatory pathway in redox balance is the Kelch-like ECH-Associating protein 1 (Keap1)nuclear factor erythroid 2 related factor 2 (Nrf2)antioxidant response element (ARE) pathway, which upregulates biosynthesis of antioxidant proteins, including antioxidant enzymes such as heme oxygenase-1 (HO-1), glutathione S -transferase, NAD(P)H:quinone oxidoreductase 1 (NQO1), glutathione reductase (GR), Trx-R, CAT, and SOD, but also nonenzymatic antioxidant proteins, e.g., Trx or anti-apoptotic proteins from Bcl-2 family 64
While subcutaneous injection into the lower limbs may be possible, it is generally not recommended as a preferred injection site due to the possible complications involved including tissue damage and injection site reactions: The skin in the lower limbs is typically thinner and more sensitive than other parts of the body, which can increase the risk of pain, bleeding, and infection
Patients who consistently follow their prescribed protocol along with proper exercise and healthy eating habits may begin seeing results within 30 days [15]
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