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Antibodies used were as follows: FoxO3a (Cell Signaling Technology, #12829), phospho-FoxO3a (Ser253) (HUABIO, ST49-01), Atrogin-1 (Proteintech, 67172-1-Ig, RRID: AB_2882468), MuRF-1 (Proteintech, 55456-1-AP, RRID: AB_11182630), GAPDH (Proteintech, 60004-1-Ig, RRID: AB_2107436), and HRP-conjugated secondary antibodies (Proteintech, SA00001-1/SA00001-2)

Number of cycles A cycle is a defined treatment period with a protocol length that varies by peptide: CJC/Ipamorelin and Tesamorelin run 12-week (3-month) cycles, the Wolverine Stack (BPC-157/TB-500) runs on a 3-months-on, 2-months-off schedule, and GHK-Cu runs 30-day cycles

However, a thorough medical consultation and blood tests are essential before starting treatment

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