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Interestingly, N-acetylcysteine, acting as an antioxidant, reinstates the mTORC1 activity (Zhang T

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To increase activity, we transitioned to PACE using a more stringent circuit, replacing the T7 RNAP promoter with the weaker T3 RNAP promoter 50 and adjusting RBS strength in front of gIII to reduce transcriptional activation following RNAP liberation (Extended Data Fig

Extracts of the medium were incubated at 80 C for at least 15 min before being centrifuged at 15,000 g for 20 min

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