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To obtain additional restraints for structural model determination of the p53 TAD2 -FOXO4 FH complex, we determined paramagnetic relaxation enhancements (PREs) for a set of 7 complexes containing spin-labeled (SL) samples ( 15 N-labeled FOXO4 FH + S46C-SL p53 TAD2 , 15 N-labeled FOXO4 FH + Q52C-SL p53 TAD2 , 15 N-labeled p53 TAD2 + Q112C-SL FOXO4 FH , 15 N-labeled p53 TAD2 + D140C-SL FOXO4 FH , 15 N-labeled p53 TAD2 + N145C-SL FOXO4 FH , 15 N-labeled p53 TAD2 + S159C-SL FOXO4 FH , 15 N-labeled p53 TAD2 + S162C-SL FOXO4 FH )
E., Dohna-Schwake, C., Hanssler, L., Neudorf, U., Hoyer, P
Consequently, the system shifts from a repair-oriented mode to a cell death mode, during which HSF1 may participate in pro-apoptotic transcriptional programs to actively eliminate irreversibly damaged germ cells ( 4 Clinical diagnosis and treatment strategies for male infertility 4.1 Diagnostic strategies No specific criteria are currently available to diagnose heat stress-induced male infertility
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