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Growth hormone is released by the anterior pituitary in pulsatile bursts predominantly during slow-wave sleep and after exercise rather than as a steady infusion

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Certain muscle groups observed in murine models, including the rectus abdominis, psoas major , and paraspinal muscles, showed a more pronounced increase in muscle density and volume, as well as a reduction in intramuscular fat content, than those observed in controls

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Pre-bed timing is the most common single-dose window because it amplifies the natural nocturnal GH pulse