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Surgical procedures for 1-week oIGF-1 infusion To test the chronic effects of oIGF-1 on fetal growth and anabolic signaling pathways, pregnant Columbia-Rambouillet mixed-breed sheep (n = 6) carrying twin fetuses underwent maternal laparotomy and hysterotomy under general anesthesia for fetal catheter placement as previously described (Rozance et al., 2018
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