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coli BL21 (DE3) (Sigma-Aldritch) at 110-fold coverage of the library size, and following 1-hr recovery from transformation, cells were immediately diluted into LB media (Gibco) containing kananycin (selecting for presence of the library) and 10 uM IPTG (inducer for library expression), beginning the massively parallel inhibition measurements
Transient palpitations: It may be related to sympathetic nerve excitation caused by the activation of 3-AR, with an incidence rate of less than 5%
It plays a critical role in neutralizing free radicals and supporting natural detoxification pathways
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