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Indeed, conducting NTA titration in 5 mM HEPES, pH 7.4 we observed that absence of 50 mM NaCl caused fivefold increase of the K D for CuHSA, which is, however, apparent and should be corrected by using of K D value for Cu(II)NTA complex at low ionic strength
the lipidated peptide is soluble in aqueous buffer at neutral pH
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Ultimately, for most research into the regenerative, anti-inflammatory, and ECM-remodeling effects attributed to GHK, the GHK-Cu complex is the active molecular entity
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