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HCV-induced ROS play a significant role in HCV pathogenesis, as Kupffer cell activation contributes to pro-fibrotic cytokine and ROS production, leading to hepatic damage 91,92

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Protein concentration was determined using a BCA kit (Thermo Scientific), after which samples were diluted to 1 mg ml 1 in 5% SDS and 50 mM TEAB

A key factor underlying the global rise in diabetes is the increasing prevalence of obesity, which is a significant risk factor for diabetes

Cerebrolysin is a brain-derived peptide mixture used for neuroprotection and cognitive enhancement