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It acts as a substrate linker for the interaction of Cullin 3 (CUL3)-dependent E3 ubiquitin ligase complex [119], with NRF2 leading to continuous ubiquitination and proteasomal degradation of NRF2 [120], thus representing the main negative regulator of NRF2 activity
When It Is Still Usable Discard immediately if: The solution is cloudy, discolored, or contains visible particles The vial has been at room temperature for over 24 hours The vial has an unusual or foul smell More than 30 days have passed since reconstitution (with bac water) More than 48 hours have passed since reconstitution (with sterile water) The solution froze at any point, even partially Likely still usable if: The solution is clear and colorless The vial has been consistently refrigerated You are within the recommended shelf life window A brief room-temperature exposure (under 4 hours) occurred once When in doubt, discard
In addition, glutathione boosts the effectiveness of other antioxidants , such as vitamin C and vitamin E
10.1016/j.clnu.2022.08.029 102
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