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After blocking for 1 h, the membranes were washed and incubated with primary antibodies at 4 C overnight

That oh my god, what what did I just do right

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Inhibition of glutaminolysis enzymes GLS1 alone may cause aberrant Th1 cell differentiation, as revealed by Johnson ( via certain amino acid transporters, but regretfully, they could not further design to support this theory

And for our folks in the audience, well mention observational trials a couple times throughout this episode