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In these models, we take into account the reaction geometry of common thiol-disulfide exchange reactions and distinguish hypothetical protein areas that might either interact with the disulfide substrate (a scaffold site) or the reducing agent (an activator site) (Fig

Avoid buffers above pH 7.5 for extended periods (maleimide hydrolyzes at alkaline pH)

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Ghrelin is one of three hormones which together act to regulate growth hormone release from the pituitary gland