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The final working concentration should be calculated according to the specific requirements of the intended in vitro research application
Tendons and ligaments heal slowly precisely because they have limited blood supply, so a compound studied for improving local vascularization draws significant research interest
Alternatively, extracellular NAD + can be utilized by NAD + glycohydrolases, generating NAM, NR, or NMN outside of the cells, which can then be transported intracellularly for NAD + synthesis 65,66
Continuing this line of research, Ackerl et al
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