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03 Draw the BAC water Draw 2.0 mL of bacteriostatic water into a syringe with a sterile drawing needle
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Essential supplies: Peptide vial (lyophilized powder) Bacteriostatic water (multi-dose vial) Insulin syringes (1 mL, 100-unit, 29-31 gauge) for drawing doses Larger syringe with needle (3 mL syringe with 18-21 gauge needle) for reconstitution Alcohol swabs or 70% isopropyl alcohol pads Clean, flat work surface Recommended additions: Disposable gloves (nitrile preferred) Sharps container for used needles Pen and label for marking the vial with date and concentration Calculator or the peptide reconstitution calculator Two notes on syringes
HPLC analysis determines peptide purity, while LC-MS confirms molecular weight and sequence accuracy
Morgan PT, Breen L
