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To obtain C2C12 single-cell colonies that incorporated the MLC enhancer for further investigation, we co-transfected the donor vector, a plasmid expressing the Cas9 protein and the sgRNAs targeting each respective site
Suitable for research protocols requiring prolonged stimulation
And while some studies have suggested that turmeric supplementation is enough to reduce total body mass index, further studies are needed to see its long-term, preventative effects on obesity
doi: 10.1080/14786419.2016.1160235 21 DahlinD
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