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A senescence -galactosidase assay was conducted using the senescence -galactosidase staining kit (Cell Signaling Technology, 9860) according to the manufacturers instructions, and then stained cells were imaged using a ZEISS Primovert inverted microscope (Carl Zeiss Microscopy)

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Next, an orthotopic K7M2 osteosarcoma model with an active immune system was established to investigate the tumor targeting and therapeutic effects of NP4 (Fig

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