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The current standard operating procedure by the European Center for the Validation of Alternative Methods (ECVAM) recommends the use of MTT reduction as parameter to make prediction on the skin irritancy of substances using EpiSkin TM test method, while IL-1 endpoint is regarded as a useful adjunct to MTT assay 48
FDA must decide whether and how to act
The mechanism is well-characterized at the biochemical level: GSH inhibits tyrosinase, the rate-limiting enzyme in melanin biosynthesis, by binding to its copper-containing active site
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Tajik N, Frech M, Schulz O, Schlter F, Lucas S, Azizov V, Rings A (2020) Targeting Zonulin and intestinal epithelial barrier function to prevent onset of arthritis
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