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In addition, it has been suggested that metformin can affect the expression of AMPK, and then mediate the decline of AKT expression, and finally play an anti-glioma cell role by inhibiting glioma stem cells

Front Pharmacol 5:151 Stamler JS, Simon DI, Osborne JA, Mullins ME, Jaraki O, Michel T, Singel DJ, Loscalzo J (1992) S-nitrosylation of proteins with nitric oxide: synthesis and characterization of biologically active compounds

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Also, the number of doses you take each day, the time allowed between doses, and the length of time you take the medicine depend on the medical problem for which you are using the medicine