Prepared solutions are generally maintained under refrigerated conditions at 36-46F (2-8C) for routine laboratory use or stored frozen for longer-term preservation, depending on the requirements of the research protocol
Because diverticula and, hence, diverticulitis can develop anywhere in the gastrointestinal tract, symptoms may mimic multiple conditions
Then, luciferin generation reagent was added to the wells and the plate was incubated 30 min at room temperature, followed by addition of a luciferin detection solution and incubation of 15 min at room temperature
The respiration buffer contained 250 mM sucrose, 0.5 mM EDTA, 15 mM KCl, 20 mM KH2PO4, and 10 mM Tris-HCl
This accomplishes several things simultaneously