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It was not until a genetically engineered strain of Pseudomonas denitrificans was used, in which eight of the genes involved in the biosynthesis of the vitamin had been overexpressed, that the complete sequence of methylation and other steps could be determined, thus fully establishing all the intermediates in the pathway
Frequency of Injections The frequency of B12 injections plays a significant role in determining how long B12 stays in the body
maintenance preserves it with less frequent dosing
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