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Before use, the protein was denatured in 6 M urea and supplemented with 2 mM tris(2-carboxyethyl)phosphine (TCEP, Sigma-Aldrich, Solna, Sweden) at pH 7.4 to obtain complete reduction and dissolution of potential aggregates
The water should run down the glass and gently dissolve the powder
PMID: 34587244
L.VetriniF.ErdinS.ErdinS.et al
Also, you won't have the needle in very long at all, just long enough for the catheter to be inserted with all the good stuff your body needs
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