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Then, water was replaced by a solution containing individual PAMPs (100 nM flg22, 50 g/mL chitin, or 2 g/mL SCFE), 100 M luminol (123072, Sigma), and 20 g/mL HRP (HY-125859, MCE), and luminescence was measured in a microplate reader (PerkinElmer) for 60 or 90 min
This is usually a localized accumulation of the injected fluid in the subcutaneous tissue
Rash and Skin Reactions Skin rashes are a known side effect and can range from mild redness or itching to more severe reactions
They also contain milk thistle extract and alpha lipoic acid to help with liver health
Standard wellness IVs run 20 to 45 minutes
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