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J Epidemiol
Non-specific antibody binding was reduced by incubating in 10% donkey serum (Sigma-Aldrich, UK) diluted in PBS for at least 30 min, followed by simultaneous incubation in primary antibodies (a melanocyte marker with a target antibody
This link between TNF- and ROS adds another dimension of complexity to the TNF signalling network, as ROS act on numerous proteins required for the regulation of cellular homeostasis, including proteins that mediate cell proliferation, survival, death, differentiation, DNA repair and metabolism 30
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