Lindqvist A, Rip J, Gaillard PJ, Bjorkman S, Hammarlund-Udenaes M
Immunofluorescence, confocal microscopy image acquisition and colocalization analysis For the immunofluorescence analysis of endogenous proteins, RAW 264.7 murine macrophages (ATCC, TIB-71) were seeded on untreated coverglass using a four-chamber 35-mm dish with 20-mm microwells (Cellvis, D35C4-20-1.5-N), incubated at 37 C for 30 min, then fixed with 3.7% paraformaldehyde for 8 min, permeabilized with 0.01% saponin and blocked with 1% BSA in PBS
The spray format offers a non-injectable option for laboratories studying sublingual peptide delivery, giving researchers an alternative route of administration to compare against traditional subcutaneous models
Synthetic peptide just means that its a chain of amino acids (the building blocks of proteins) that was created by scientists
An approach to familiar lymphedema Pediatric Children Lymphedema Primary Lymphedema French National Diagnosis and Care Protocol (PNDS)