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NaBPC157 (10 g or 10 ng/kg b.w., i.p.) or saline (5.0 ml/kg b.w., i.p.) was applied either 1 hour before or 1 hour after both carotid arteries were ligated for 3 or 6 hours in rats
If foam appears, set the vial aside for 15 minutes and let it settle before checking clarity
A common approach is to use a 1mL syringe for precision
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The study was reviewed and approved by the Ethics Committee on the Use of Animals (CEUA) of the Universidade do Extremo Sul Catarinense (UNESC), under protocol number 07/2023
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