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Western blotting Total protein was extracted from paraformaldehyde-fixed, paraffin-embedded pancreatic tissues using the FFPE Total Protein Extraction Kit (Sangon Biotech, Shanghai, China) or from cultured PSCs using RIPA Lysis Buffer (Beyotime)

These can destabilize the copper-peptide bond or cause irritation

Beyond bacteria, ChaC-like proteins play roles in diverse pathogens

Graber, M

Cell Proliferation, Differentiation, and Adhesion In cellular models, GHK-Cu is assessed for its influence on cell-matrix interactions, integrin-related signaling, and adhesion dynamics, supporting research into how peptide-copper complexes affect tissue organization in epithelial and mesenchymal systems